c1 single cell auto prep system microfluidic platform  (fluidigm)

Journal: Blood Advances

Article Title: Single-cell analysis reveals selection of TP53 -mutated clones after MDM2 inhibition

doi: 10.1182/bloodadvances.2021005867

Figure Lengend Snippet: Clonal architecture of TP53 -mutated MPN patients. Phylogenetic trees show the order of mutation acquisition during MPN history with maximum likelihood in 8 patients. The mutational history was manually reconstructed from the single-cell data. In the case of MPN3, it was not possible to infer the order of mutation acquisition in JAK2 and U2AF1 because of the absence of cells with wild-type version of these genes. Each circle represents a clone identified in single-cell analysis using Tapestri platform and Tapestry Insights software (Mission Bio). The size of each circle denotes the relative clone size. The nomenclature of each mutation is indicated only when 2 or more mutations were present in the same gene.

Article Snippet: Clonal architecture was determined using the single-cell high-throughput microfluidic Tapestri platform coupled to the Tapestri Insights bioinformatics software (Mission Bio).

Techniques: Mutagenesis, Single-cell Analysis, Software

Journal: Biological Psychiatry Global Open Science

Article Title: Single-Cell RNA Sequencing and Its Applications in the Study of Psychiatric Disorders

doi: 10.1016/j.bpsgos.2022.03.013

Figure Lengend Snippet: Microfluidics device for single-cell and barcoded bead encapsulation in oil emulsion.

Article Snippet: STRT-seq was modified to include the commercially available C1 Single-Cell Auto Prep System microfluidic platform (Fluidigm) and became STRT/C1 , which includes UMI tags in the oligo-dT used for second strand cDNA synthesis ( , right panel).

Techniques: Encapsulation, Emulsion